Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reactions
نویسندگان
چکیده
منابع مشابه
Enzyme treatment-free and ligation-independent cloning using caged primers in polymerase chain reactions.
A new simple scheme for constructing recombinant vectors that does not require any restriction enzyme, ligase, or any other special enzyme treatment has been developed. By using caged primers in PCR, unnatural sticky-ends of any sequence, which are sufficiently long for ligation-independent cloning (LIC), are directly prepared on the product after a brief UVA irradiation. Target genes and vecto...
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ژورنال
عنوان ژورنال: Molecules
سال: 2011
ISSN: 1420-3049
DOI: 10.3390/molecules17010328